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Neutrons observe vitamin B6-dependent enzyme activity useful for drug development

ORNL鈥檚 Steven Dajnowicz (left) and Andrey Kovalevsky prepared a sample to begin neutron structural analysis of a vitamin B6-dependent protein using the IMAGINE beamline at ORNL鈥檚 High Flux Isotope Reactor. Results of the study could open avenues for new antibiotics and drugs to battle diseases. Credit: Genevieve Martin/Oak Ridge National Laboratory, U.S. Dept. of Energy
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OAK RIDGE, Tenn., Oct. 16, 2017 鈥� Scientists at the Department of Energy鈥檚 Oak Ridge National Laboratory have performed neutron structural analysis of a vitamin B6-dependent protein, potentially opening avenues for new antibiotics and drugs to battle diseases such as drug-resistant tuberculosis, malaria and diabetes.

Specifically, the team used neutron crystallography to study the location of hydrogen atoms in aspartate aminotransferase, or AAT, an enzyme vital to the metabolism of certain amino acids.

鈥淲e visualized the first neutron structure of a vitamin B6 enzyme that belongs to a large protein family with hundreds of members that exist in nature,鈥� said ORNL鈥檚 Andrey Kovalevsky, a senior co-author of the study, which in Nature Communications.

Vitamin B6-dependent proteins are part of a diverse group of enzymes that conduct over a hundred different chemical reactions in cells. The enzymes are of interest to biomedical, as well as bioenergy, researchers because of their role in metabolizing amino acids and other cell nutrients.

鈥淭hese enzymes are unique in that each one performs a specific chemical reaction with exquisite accuracy, while suppressing other viable chemical transformations,鈥� Kovalevsky said. 鈥淗ow they accomplish this is not well understood, but it is of great significance for drug design.鈥�

The team鈥檚 previous research predicted that hydrogen atoms move in and around the enzyme鈥檚 active site, where the chemical reaction takes place, indicating that the hydrogen atoms鈥� positioning controls the reaction type. Knowing the precise location of hydrogen atoms can explain why the behavior of these enzymes is so specific, but hydrogen is hard to detect with standard methods such as X-ray crystallography.

To directly determine the positions of hydrogen atoms within AAT, the ORNL-led team turned to neutron diffraction techniques. The researchers exposed delicate protein crystals to neutrons using the IMAGINE beamline at ORNL鈥檚 High Flux Isotope Reactor and the LADI-III beamline at the Institut Laue-Langevin in Grenoble, France.

Surprisingly, the team observed a reaction within one AAT protein biomolecule while another AAT biomolecule was unchanged, providing a before-and-after perspective of the enzyme-catalyzed chemical reaction.

鈥淭he data revealed that in one of the enzyme鈥檚 biomolecular structures the covalent bonds reorganized after a chemical reaction occurred in the active site and, in another, the reaction had not taken place,鈥� Kovalevsky said. 鈥淓ssentially, we were able to obtain two structures in one crystal, which has never been done before for any protein using neutrons.鈥�

With this knowledge, the team will run molecular simulations to determine the hydrogen atoms鈥� specific behavior when interacting with the enzyme. The results could be useful in guiding the future design of novel medicines against multidrug-resistant tuberculosis, malaria, diabetes and antibiotic-resistant bacteria.

鈥淭his study highlights how neutrons are an unrivaled probe for identifying the location of hydrogen atoms in biological systems, providing us with an unprecedented level of structural detail for this important enzyme,鈥� LADI-III beamline scientist Matthew Blakeley said.

The neutron crystallography study, titled 鈥淒irect visualization of critical hydrogen atoms in a pyridoxal 5鈥�- phosphate enzyme,鈥� included co-authors Steven Dajnowicz of ORNL and the University of Toledo; ORNL鈥檚 Andrey Kovalevsky, Jerry Parks, Ryne C. Johnston and Kevin L. Weiss; Matthew P. Blakeley of the Institut Laue Langevin; David A. Keen of Rutherford Appleton Laboratory; Oksana Gerlits of the University of Tennessee, and Timothy C. Mueser of the University of Toledo.

Steven Dajnowicz, the paper鈥檚 lead co-author and a doctoral student at the University of Toledo, works in ORNL鈥檚 Biology and Soft Matter Division as part of ORNL鈥檚 Graduate Opportunities (GO!) student program. Mueser is Dajnowicz鈥� advisor and a senior co-author of the paper.

This project was funded by DOE鈥檚 Office of Science and used resources at ORNL鈥檚 Center for Structural Molecular Biology. Neutron crystallography work was performed at ORNL鈥檚 HFIR, a DOE Office of Science User Facility, and at . This work also used ORNL鈥檚 Compute and Data Environment for Science for quantum mechanical calculations. This project was also supported in part by the Center for the Advancement of Science in Space and the National Aeronautics and Space Administration.

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